RNA-Seq data analysis

Organizer:

Dr. Kolsoum Inanloo

Tehran University 

 

:Goal 

  •  Introduction to RNA-Seq
  • RNA-Seq applications
  • RNA-Seq data analysis

   

:Applicants

  • Genetic Students
  • Cell and molecular Biology students
  • Bioinformatic students 


RNA Sequencing Workshop

-Introduction to NGS

-NGS platforms and instruments

-Application of NGS in biology and medicine

-Project Design and Experimental Considerations

-Laboratory techniques (RNA extraction, Quality control, cDNA Library preparation) 

-Introduction to the Linux terminal

-Quality Control and Data Pre-processing

-Mapping to a Reference Genome and transcriptome (STAR)

-Transcript expression quantification (Cuffdiff, HTSeq)

-Differential Expression Analysis (DESeq2)

-Post differential expression analysis (gene set enrichment analysis, Gene Ontology/Pathway analysis, Generation of figures and tables)




Key Speakers


Conference organizers




Sponsors



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